Device

Part:BBa_K5477047:Design

Designed by: Kate Malana Escobar, Victor Bay   Group: iGEM24_UCopenhagen   (2024-09-29)


Detoxification device against dioxin and PCBs


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 7148
    Illegal PstI site found at 232
    Illegal PstI site found at 1434
    Illegal PstI site found at 2548
    Illegal PstI site found at 2745
    Illegal PstI site found at 3129
    Illegal PstI site found at 3569
    Illegal PstI site found at 3629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 7148
    Illegal PstI site found at 232
    Illegal PstI site found at 1434
    Illegal PstI site found at 2548
    Illegal PstI site found at 2745
    Illegal PstI site found at 3129
    Illegal PstI site found at 3569
    Illegal PstI site found at 3629
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 7148
    Illegal BglII site found at 5440
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 7148
    Illegal PstI site found at 232
    Illegal PstI site found at 1434
    Illegal PstI site found at 2548
    Illegal PstI site found at 2745
    Illegal PstI site found at 3129
    Illegal PstI site found at 3569
    Illegal PstI site found at 3629
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 7148
    Illegal PstI site found at 232
    Illegal PstI site found at 1434
    Illegal PstI site found at 2548
    Illegal PstI site found at 2745
    Illegal PstI site found at 3129
    Illegal PstI site found at 3569
    Illegal PstI site found at 3629
    Illegal NgoMIV site found at 326
    Illegal NgoMIV site found at 413
    Illegal NgoMIV site found at 2951
    Illegal NgoMIV site found at 3070
    Illegal AgeI site found at 1956
    Illegal AgeI site found at 6146
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In our system, we have integrated two detoxification modules in yeast to create a highly efficient platform for the removal of environmental pollutants, including polycyclic aromatic hydrocarbons (PAHs), dioxins, and polychlorinated biphenyls (PCBs). These modules, CYP1A1-pGAL1/10-POR and UDPD-pGAL1/10-UGT1A1, work in tandem under the control of the pGAL1/10 bidirectional promoter, which allows for the coordinated expression of key enzymes in opposite directions.



Source

Homo sapiens and Saccharomyces cerevisiae

References